| Product Name | 3BP2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of 3BP2 of human origin |
| Modification Notes | 3BP2 is a Syk family kinase-interacting protein (SKIP) that is expressed in spleen and peripheral blood leukocytes. 3BP2 was originally characterized as an Abl SH3-interacting protein, as it contains a single proline-rich domain and an SH2 domain, consistent with other adaptor molecules. In Jurkat T cells transfected with 3BP2, stimulation of T cell receptors (TCR) rapidly induces the redistribution of 3BP2 from the cytoplasm to the membrane, where it associates with the TCR protein tyrosine kinase complexes. Through this translocation, 3BP2 is able to selectively bind to Flt3/Flk2 receptors and to the phosphorylated Syk, LAT and ZAP-70 proteins. In T lymphocytes, the overexpression of 3BP2, specifically the overexpression of the SH2 and proline rich domains, is sufficient to induce the activation of several transcription factors, including NFAT and AP-1. This transactivation results in the upregulation of the IL-2 gene promoter and suggests a role for 3BP2 in mediating T cell signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 3BP2 long and short isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of 3BP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of 3BP2 of human origin |
| Entrez Gene | 6452 |
|---|
| Application Notes | recommended for detection of 3BP2 long and short isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
