Application
Western blot analysis of PKC θ phosphorylation in PMA treated (A) and PMA and lambda protein phosphatase treated (B) Jurkat whole cell lysates.Application
Antibodies tested include p-PKC θ (Ser 676): (A,B,C) and PKC θ (C-18): (D,E,F).| Product Name | p-PKC θ Antibody (Ser 676) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 676 of PKCθ of mouse origin |
| Modification | Phosphoralated |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (å, ∫I, ∫II and ©) and novel (n) PKC isoforms (∂, é , Ω, h and †). PKC isoforms can be activated through tyrosine phosphorylation and catalytically activated upon treatment with H2O2. The Tyr 155, 525, 523 and 565 residues in the catalytic domain are crucial for activation of these enzymes. The residue Ser 643 appears to be an autophosphorylation site. PKC theta can undergo autophosphorylation on serine 676 (Ser 676) in the turn loop and serine 695 (Ser 695) in the hydrophobic loop. Phosphorylation of serine 676 may negatively regulate activation of NF-kappa B. Ser-695 is crucial to activate the phosphorylation threonine 692 (Thr 692) and threonine 703 (Thr 703) residues, both of which are necessary for mobility shift. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 676 phosphorylated p-PKCθ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 676 of PKC&theta of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 676 of PKCθ of mouse origin |
| Entrez Gene | 5588 |
|---|
Application
Western blot analysis of PKC θ phosphorylation in PMA treated (A) and PMA and lambda protein phosphatase treated (B) Jurkat whole cell lysates.
Application
Antibodies tested include p-PKC θ (Ser 676): (A,B,C) and PKC θ (C-18): (D,E,F).| Application Notes | recommended for detection of Ser 676 phosphorylated p-PKCθ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
