Application
Western blot analysis of CD163 expression in THP-1 (A) and CCRF-CEM (B) whole cell lysates.| Product Name | CD163 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1027-1156 mapping at the C-terminus of CD163 of human origin |
| Modification Notes | CD163, also designated M130, is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on macrogphages and to a lesser extent on monocytes. The acute phase-regulated and signal-inducing macrophage protein, CD163, is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 binds only haptoglobin and hemoglobin in complex, which indicates the exposure of a receptor-binding neoepitope. The receptor-ligand interaction is calcium-dependent and of high affinity. The existence of several CD163 isoforms, which differ in the structure of their cytoplasmic domains and putative phosphorylation sites, suggests that these isoforms also differ in their signaling mechanism. The gene which encodes CD163 maps to human chromosome 12p13.31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD163 isoforms a and b of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1027-1156 mapping at the C-terminus of CD163 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1027-1156 mapping at the C-terminus of CD163 of human origin |
| Entrez Gene | 9332 |
|---|
Application
Western blot analysis of CD163 expression in THP-1 (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of CD163 isoforms a and b of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
