Application
Western blot analysis of HoxB4 expression in non-transfected: (A) and human HoxB4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HoxB4 expression in Jurkat whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | HoxB4 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 91-140 mapping within an internal region of HoxB4 of human origin |
| Modification Notes | The homeobox genes encode a family of transcription factors that regulate development and postnatal tissue homeostasis. Encoded by the HOXB4 gene, the nuclear phosphoprotein HoxB4 plays a key role in regulating the balance between hematopoietic stem cell renewal and differentiation. Hematopoietic expression of HoxB4 is regulated by the binding of USF-1 and USF-2, the binding of which may be favored by cytokines promoting stem cell self-renewal versus differentiation. HoxB4 is dependent on AP-1 expression to induce changes in cellular proliferation and differentiation, which increases the levels of cyclin D1, thereby linking HoxB4 with key elements of the cell cycle machinery. HoxB4 also participates in the down-regulation of c-Myc expression. It is expressed in developing hair follicles as well as in K-562 and HL-60 cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HoxB4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 91-140 mapping within an internal region of HoxB4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 91-140 mapping within an internal region of HoxB4 of human origin |
| Entrez Gene | 3214 |
|---|
Application
Western blot analysis of HoxB4 expression in non-transfected: (A) and human HoxB4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HoxB4 expression in Jurkat whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of HoxB4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
