Application
Western blot analysis of phosphorylated Rac 1 expression in untreated (A) and PMA-treated (B) 293 cell extracts.| Product Name | p-Rac 1 Antibody (Ser 71) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A large number of low molecular weight GTP-binding proteins of the Ras superfamily have been identified in eukaryotic cells; they regulate many fundamental processes such as cell growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. Rac 1 is activated in a type I interferon (IFN) dependent manner; its function is required for downstream engagement of the p38 MAP kinase pathway. The p38 MAP kinase plays an essential role in IFN-dependent transcriptional regulation. The serine/threonine kinase Akt, of the phospho-inositide 3-kinase signal transduction pathway phosphorylates serine 71 of Rac 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 71 phosphorylated Rac 1 of mouse rat and human origin by WB and IP |
|---|---|
| Tissue Specificity | recommended for detection of Ser 71 phosphorylated Rac 1 of mouse, rat and human origin by WB and IP |
| Entrez Gene | 5879 |
|---|
Application
Western blot analysis of phosphorylated Rac 1 expression in untreated (A) and PMA-treated (B) 293 cell extracts.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
