Application
Western blot analysis of CIB expression in non-transfected: (A) and mouse CIB transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CIB expression in non-transfected: (A) and mouse CIB transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CIB expression in human platelet whole cell lysate.| Product Name | CIB Antibody (H-115) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 77-191 mapping at the C-terminus of CIB of human origin |
| Modification Notes | Platelets regulate the function of Integrin α2b/β3 (GPIIb/IIIa), the platelet Fibrinogen receptor, which is involved in the binding of proteins to integrin cytoplasmic domains. A novel protein, CIB, for calcium- and integrin-binding protein (also designated as Kip for kinase interacting protein, SIP2-28 and DNA-PKcs interacting protein), binds specifically at the cytoplasmic domain of α2b by a number of positively charged residues in its binding site. Binding of CIB to the α2b is affected by fluctuations in the intracellular calcium concentration. In aggregated platelets, endogenous CIB and α2b/β3 translocate to the Triton X-100-insoluble cytoskeleton, demonstrating that the cellular localization of CIB is regulated. CIB also binds to DNA-PKcs, which is a nu-clear protein serine/threonine kinase that plays a role in the DNA repair and recombination process of lymphoid development. Fnk also binds to the CIB, suggesting that CIB may be a regulatory subunit of polo-like kinases. CIB shows significant homology to calcineurin B and calmodulin, and its mRNA levels are ubiquitously expressed in various human tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CIB of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 77-191 mapping at the C-terminus of CIB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 77-191 mapping at the C-terminus of CIB of human origin |
| Entrez Gene | 10519 |
|---|
Application
Western blot analysis of CIB expression in non-transfected: (A) and mouse CIB transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CIB expression in non-transfected: (A) and mouse CIB transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CIB expression in human platelet whole cell lysate.| Application Notes | recommended for detection of CIB of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
