| Product Name | Asef Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The adenomatous polyposis coli gene (APC) is mutated in familial adenomatous polyposis and in sporadic colorectal tumors. Familial adenomatous polyposis is a dominantly inherited disease characterized by multiple adenomatous polyps in the colon The human full-length Asef cDNA encodes a protein of 619 amino acids with motifs found in the Dbl family of proteins. The APC gene product binds through its armadillo repeat domain to a Rac-specific guanine nucleotide exchange factor (GEF), termed APC-stimulated guanine nucleotide exchange factor (Asef). Endogenous APC has been shown to co-localize with Asef in mouse colon epithelial cells and neuronal cells. Furthermore, APC has been shown to enhance the GEF activity of Asef and stimulate Asef-mediated cell flattening, membrane ruffling and lamellipodia formation in MDCK cells, suggesting that the APC-Asef complex may regulate the actin cytoskeletal network, cell morphology and migration, and neuronal function. Full-length APC protein is located in both the membrane/cytoskeletal and the nuclear fractions . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Asef isoforms 1, 2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 518-657 mapping at the C-terminus of Asef of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 518-657 mapping at the C-terminus of Asef of human origin |
| Entrez Gene | 50649 |
|---|
| Application Notes | recommended for detection of Asef isoforms 1, 2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
