Application
Western blot analysis of CPSF4 expression in Jurkat whole cell lysate.| Product Name | CPSF4 Antibody (E-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CPSF4 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | CPSF4 (Cleavage and polyadenylation specificity factor subunit 4) is a nuclear protein that belongs to the CPSF4/YTH1 family and contains five C3H1-type zinc fingers and one CCHC-type zinc finger. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation. CPSF is a multisubunit factor consisting of four subunits. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. The largest subunit of CPSF can, by itself, bind preferentially to AAUAAA-containing RNAs and binds specifically to both the suppressor of forked subunit of the cleavage stimulatory factor (CstF) and to poly (A) polymerase. snRNPA protein (U1 snRNP-A) interacts with and affects the activity of CPSF by stabilizing the interaction of CPSF with the AAUAAA-containing RNAs to increase the efficiency of polyadenylation. Efficient processing of 3’ core poly(A) site also requires specific sequences located 76 nucleotides upstream of the AAUAAA hexamer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CPSF4 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of CPSF4 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 10898 |
|---|
Application
Western blot analysis of CPSF4 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
