Application
Western blot analysis of GABAB R1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat synaptic membrane tissue extracts.| Product Name | p-GABAB R1 Antibody (Ser 923) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 923 phosphorylated GABAB R1 isoform 1A of rat origin, correspondingly Ser 954 phosphorylated GABAB R1 isoform 1E of rat origin, correspondingly Ser 924 phosphorylated GABAB R1 of human origin and correspondingly Ser 923 pho |
| Modification | Phosphoralated |
| Modification Notes | In the central nervous system (CNS), gamma-aminobutyric acid (GABA) is the main main inhibitory neurotransmitter that functions to regulate neuronal firing. GABA exerts its effects through two different kinds of receptors: ionotropic receptors (GABAA R and GABAC R), which produce fast inhibitory signals, and metabotropic receptors (GABAB R), which produce slow inhibitory signals. The GABAB R receptor is a heterodimer that consists of two multi-pass membrane proteins, designated GABAB R1 and GABAB R2, both of which belong to the G protein-coupled receptor family and are highly expressed in brain tissue. Together, GABAB R1 and GABAB R2 play a crucial role in the fine-tuning of inhibitory synaptic transmissions and are implicated in slow wave sleep, muscle relaxation, hippocampal long-term potentiation and antinociception events. Both GABAB R1 and GABAB R2 are regulated by G proteins that have a variety of functions, including activation of potassium channels, inhibition of adenylyl cyclase (A cyclase) activity and modulation of inositol phospholipid hydrolysis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 923 phosphorylated GABAB R1 isoform 1A of rat origin correspondingly Ser 954 phosphorylated GABAB R1 isoform 1E of rat origin correspondingly Ser 924 phosphorylated GABAB R1 of human origin and correspondingly Ser 923 pho |
|---|---|
| Tissue Specificity | recommended for detection of Ser 923 phosphorylated GABAB R1 isoform 1A of rat origin, correspondingly Ser 954 phosphorylated GABAB R1 isoform 1E of rat origin, correspondingly Ser 924 phosphorylated GABAB R1 of human origin and correspondingly Ser 923 phosphorylated GABAB R1 of mouse origin by WB and IP; also reactive with additional species, including bovine |
| Entrez Gene | 2550 |
|---|
Application
Western blot analysis of GABAB R1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat synaptic membrane tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
