Application
Western blot analysis of TFB1M expression in 721B whole cell lysate.| Product Name | TFB1M Antibody (X-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TFB1M of human origin by WB, IP and ELISA |
| Modification Notes | TFB1M (transcription factor B1, mitochondrial), also known as CGI75, mtTFB or CGI-75, is a 346 amino acid mitochondrial protein that, along with mtTFA and MtRPOL, is required for the transcription of genes from mitochondrial DNA. Expressed ubiquitously, TFB1M functions as an S-adenosyl-L-methionine-dependent methyltransferase that specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop and is required for mitochondrial DNA transcription. Variations in the gene encoding TFB1M are thought to influence the pathogenesis of aminoglycoside-induced deafness (AID), a disorder that is characterized by hearing loss and is caused by irregular methylation of 12S rRNA. The gene encoding TFB1M maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TFB1M of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TFB1M of human origin by WB, IP and ELISA |
| Entrez Gene | 51106 |
|---|
Application
Western blot analysis of TFB1M expression in 721B whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
