Application
Western blot analysis of Unc18-3 expression in non-transfected: (A) and human Unc18-3 transfected: (B) 293T whole cell lysates.| Product Name | Unc18-3 Antibody (H-96) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 50-145 mapping near the N-terminus of Unc18-3 of human origin |
| Modification Notes | Unc18-1, 2, and 3 (syntaxin binding proteins 1-3, STXBP1-3, UNC18-a-c, MUNC18-1-3) are chaperone molecules that block syntaxin interactions with cognate SNARE (soluble NSF attachment protein (SNAP) receptor) proteins and regulate exocytosis. Unc18-1-3 mRNA is present in RBL-2H3 mast cells, mouse bone marrow derived mast cells (BMMC), and platelets. Unc18-1 Ser-313 is a protein kinase C phosphorylation site and Thr-574 is a cyclin-dependent kinase 5 phosphorylation site that regulates Unc18-1/syntaxin1A interactions. Unc18-1 is phosphorylated on Ser-313 in response to phorbol ester treatment in adrenal chromaffin cells. Unc18-2 colocalizes with syntaxin 3 at the apical plasma membrane of intestinal, proximal tubule, and collecting duct epithelial cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Unc18-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 50-145 mapping near the N-terminus of Unc18-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 50-145 mapping near the N-terminus of Unc18-3 of human origin |
| Entrez Gene | 6814 |
|---|
Application
Western blot analysis of Unc18-3 expression in non-transfected: (A) and human Unc18-3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Unc18-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
