Application
Western blot analysis of HAX-1 expression in non-transfected: (A) and human HAX-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HAX-1 expression in non-transfected 293T: (A), human HAX-1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.Application
Western blot analysis of HAX-1 expression in A-673 nuclear extract (A) and A-673 (B), MES-SA/Dx5 (C), SK-MEL-24 (D) and Saos-2 (E) whole cell lysates.| Product Name | HAX-1 Antibody (FL-279) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-279 representing full length HAX-1 of human origin |
| Modification Notes | HAX-1 (HS1-associated protein X-1 or HS1-binding protein protein X-1), encodes a novel protein. HAX-1 has previously been shown to associate with HS1, a protein specifically expressed in cells of the hematopoietic lineage, and is thought to be involved in signal transduction in B cells and apoptosis. Though first identified as a protein that associates with HS1, recent data has also revealed interactions between HAX-1 and three disparate proteins: Polycystin-2 (derived from the PKD2 gene), a protein linked to polycystic kidney disease; Cortactin; and EBNA-LP (Epstein-Barr virus nuclear antigen leader protein). Additionally, HAX-1 has been identified as a binding partner to the carboxy-terminus of the K15 protein of Kaposi’s sarcoma-associated herpesvirus. K15 interacts with cellular HAX-1 in vitro and in vivo. Furthermore, HAX-1 co-localizes with K15 in the endoplasmic reticulum and mitochondria. Immunofluorescence experiments show that in most cells PKD2 and HAX-1 co-localize in the cell body, but in some cells PKD2 and HAX-1 also are sorted into cellular processes and lamellipodia. The HAX-1 gene is expressed ubiquitously among tissues. Its protein is localized mainly in mitochondria, but also in endoplasmic reticulum and the nuclear envelope of the cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HAX-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-279 representing full length HAX-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-279 representing full length HAX-1 of human origin |
| Entrez Gene | 10456 |
|---|
Application
Western blot analysis of HAX-1 expression in non-transfected: (A) and human HAX-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HAX-1 expression in non-transfected 293T: (A), human HAX-1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.
Application
Western blot analysis of HAX-1 expression in A-673 nuclear extract (A) and A-673 (B), MES-SA/Dx5 (C), SK-MEL-24 (D) and Saos-2 (E) whole cell lysates.| Application Notes | recommended for detection of HAX-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
