Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.Application
Western blot analysis of cytochrome b5 expression in rat liver (A) and mouse liver (B) tissue extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of cytochrome b5 expression in 293T whole cell lysate.Application
Western blot analysis of cytochrome b5 expression in Hep G2 whole cell lysate (A) and mouse liver tissue extract (B).| Product Name | cytochrome b5 Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-134 mapping at the C-terminus of cytochrome b5 of human origin |
| Modification Notes | Cytochrome b5 is a membrane-bound member of the cytochrome b family. A heme protein that functions as an electron carrier for many membrane-bound oxygenases, cytochrome b5 possesses two heme groups, which are not covalently attached to the protein. Two isoforms of cytochrome b5, a microsomal membrane-bound form and a cytoplasmic form, are produced by alternative splicing. Mutations in cytochrome b5 are associated with Leber’s hereditary optic neuropathy and with myopathy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cytochrome b5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-134 mapping at the C-terminus of cytochrome b5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-134 mapping at the C-terminus of cytochrome b5 of human origin |
| Entrez Gene | 1528 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.
Application
Western blot analysis of cytochrome b5 expression in rat liver (A) and mouse liver (B) tissue extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of cytochrome b5 expression in 293T whole cell lysate.
Application
Western blot analysis of cytochrome b5 expression in Hep G2 whole cell lysate (A) and mouse liver tissue extract (B).| Application Notes | recommended for detection of cytochrome b5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
