Application
Western blot analysis of Mel-CAM expression in non-transfected: (A) and human Mel-CAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Mel-CAM expression in non-transfected 293T: (A), human Mel-CAM transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Mel-CAM expression in non-transfected: (A) and mouse Mel-CAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Mel-CAM expression in HeLa whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of Mel-CAM expression in HUV-EC-C (A) and SK-MEL-24 (B) whole cell lysates.| Product Name | Mel-CAM Antibody (H-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 585-646 mapping within a C-terminal cytoplasmic domain of MelCAM of human origin |
| Modification Notes | The tumorigenic and metastatic phenotype of melanoma cells correlates well with an increased expression of cell-cell and cell-matrix adhesion receptors. The human Mel-CAM gene encodes a transmembrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. The deduced human sequence of 603 amino acids consists of a signal peptide, five immunoglobulin-like domains, a transmembrane region and a short cytoplasmic tail. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human mela-nomas and correlates well with development of metastatic disease. Mel-CAM activation initiates an outside-in signaling pathway that involves the protein tyrosine kinases Fyn, FAK and paxillin. Mel-CAM influences the dynamics of Actin cytoskeleton rearrangement and is essential for the maintenance of thymic architecture and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MelCAM of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 585-646 mapping within a C-terminal cytoplasmic domain of MelCAM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 585-646 mapping within a C-terminal cytoplasmic domain of MelCAM of human origin |
| Entrez Gene | 4162 |
|---|
Application
Western blot analysis of Mel-CAM expression in non-transfected: (A) and human Mel-CAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Mel-CAM expression in non-transfected 293T: (A), human Mel-CAM transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Mel-CAM expression in non-transfected: (A) and mouse Mel-CAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Mel-CAM expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of Mel-CAM expression in HUV-EC-C (A) and SK-MEL-24 (B) whole cell lysates.| Application Notes | recommended for detection of MelCAM of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
