Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of RBP expression in non-transfected: (A) and human RBP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RBP expression in Hep G2 whole cell lysate.Application
Western blot analysis of RBP expression in MDCK (A) and MES-SA/Dx5 (B) whole cell lysates.| Product Name | RBP Antibody (FL-201) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-201 representing full length RBP of human origin |
| Modification Notes | Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature RBP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-201 representing full length RBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-201 representing full length RBP of human origin |
| Entrez Gene | 5950 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of RBP expression in non-transfected: (A) and human RBP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RBP expression in Hep G2 whole cell lysate.
Application
Western blot analysis of RBP expression in MDCK (A) and MES-SA/Dx5 (B) whole cell lysates.| Application Notes | recommended for detection of precursor and mature RBP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
