Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of versican expression in A-431 (A), JAR (B), MIA PaCa-2 (C), U-87 MG (D) and HeLa (E) whole cell lysates.| Product Name | versican Antibody (H-56) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 3341-3396 mapping at the C-terminus of versican of human origin |
| Modification Notes | Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a lectin-like sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycosaminoglycan chains. Accumulation around smooth muscle cells in lesions of atherosclerosis, suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels. The human verisican gene maps to chromosome 5q14.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of versican of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3341-3396 mapping at the C-terminus of versican of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3341-3396 mapping at the C-terminus of versican of human origin |
| Entrez Gene | 1462 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of versican expression in A-431 (A), JAR (B), MIA PaCa-2 (C), U-87 MG (D) and HeLa (E) whole cell lysates.| Application Notes | recommended for detection of versican of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
