Application
Western blot analysis of Hrs expression in CCRF-CEM whole cell lysate (A) and mouse testis tissue extract (B).Application
Western blot analysis of Hrs expression in non-transfected 293: (A), human Hrs transfected 293: (B), HEL 92.1.7 (C), AML-193 (D), K-562 (E) and M1 (F) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | Hrs Antibody (M-79) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 573-651 mapping near the C-terminus of Hrs of mouse origin |
| Modification Notes | The hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a zinc-finger protein that interacts with STAM and undergoes tyrosine phosphorylation in response to IL2, CSF2 or HGF. Hrs is involved in intracellular trafficking and signal transduction and is associated with early endosomes. Hrs contains a phosphatidylinositol 3-phosphate-binding domain that contributes to its endosomal targeting, where Hrs co-localizes with Clathrin via a Clathrin box motif at the carboxy-terminus of Hrs. Hrs is essential for ventral folding morphogenesis and shares structual similarity to the yeast protein Vps27p, which is involved in vacuolar protein sorting. The human Hrs gene, which maps to chromosome 17q25, enodes a 777 amino acid protein. In Schwann cells, Hrs co-localizes at endosomes with the tumor suppressor protein schwannomin, suggesting a role for schwannomin in Hrs-mediated cell signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Hrs of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 573-651 mapping near the C-terminus of Hrs of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 573-651 mapping near the C-terminus of Hrs of mouse origin |
| Entrez Gene | 9146 |
|---|
Application
Western blot analysis of Hrs expression in CCRF-CEM whole cell lysate (A) and mouse testis tissue extract (B).
Application
Western blot analysis of Hrs expression in non-transfected 293: (A), human Hrs transfected 293: (B), HEL 92.1.7 (C), AML-193 (D), K-562 (E) and M1 (F) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of Hrs of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
