Application
Western blot analysis of TWIK-1 expression in non-transfected: (A) and human TWIK-1 transfected: (B) 293T whole cell lysates.| Product Name | TWIK-1 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 287-336 mapping within a C-terminal cytoplasmic domain of TWIK-1 of human origin |
| Modification Notes | K+ channels are divided into three subclasses, reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS, and 2TMS. Members of the 4TMS class contain two distinct pore regions, and include TASK, TREK, TRAAK, and TWIK. TWIK-1 mRNA is expressed abundantly in brain and at lower levels in lung, kidney, and skeletal muscle. TWIK-2 shares low sequence homology with other mammalian family group members, and only 34% homology with TWIK-1. Human TWIK-2 is expressed in pancreas, placenta and heart, while mouse TWIK-2 is expressed in liver. TWIK-2 is inhibited by intracellular, but not extracellular, acidification. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TWIK-1 of h, r and, to a lesser extent, mouse origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 287-336 mapping within a C-terminal cytoplasmic domain of TWIK-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 287-336 mapping within a C-terminal cytoplasmic domain of TWIK-1 of human origin |
| Entrez Gene | 3775 |
|---|
Application
Western blot analysis of TWIK-1 expression in non-transfected: (A) and human TWIK-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TWIK-1 of h, r and, to a lesser extent, mouse origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
