Application
Western blot analysis of Calbindin D28K expression in rat small intestine tissue extract.Application
Western blot analysis of human recombinant Calbindin D28K fusion protein.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | Calbindin D28K Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 35-84 mapping near the N-terminus of Calbindin D28K of human origin |
| Modification Notes | The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 å and ∫, Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14), Calgranulin C and the Parvalbumin family members, including Parvalbumin å and Parvalbumin ∫ (also designated oncomodulin). Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261 amino acid protein with six EF-hand domains, four of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Calbindin D28K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-84 mapping near the N-terminus of Calbindin D28K of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-84 mapping near the N-terminus of Calbindin D28K of human origin |
| Entrez Gene | 793 |
|---|
Application
Western blot analysis of Calbindin D28K expression in rat small intestine tissue extract.
Application
Western blot analysis of human recombinant Calbindin D28K fusion protein.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of Calbindin D28K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
