Application
Western blot analysis of CRX expression in non-transfected: (A) and mouse CRX transfected: (B) whole cell lysates.Application
Western blot analysis of CRX expression in non-transfected: (A) and mouse CRX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRX expression in Y79 (A) and ARPE-19 (B) whole cell lysates.IF
(C) Immunocytochemistry analysis was used to detect cone-rod homeobox (Crx)-positive cells with different treatments (n = 3).| Product Name | CRX Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The cone-rod homeobox-containing gene (CRX) encodes a transcription factor that coordinates the expression of several photoreceptor genes in the developing retina, including opsin and rhodopsin. Specifically, CRX binds the OTX motif (TAATCC/A) upstream from photoreceptor genes. The CRX gene is also expressed in the pinealocytes of the pineal gland and may regulate pineal circadian activity by controling the expression of melatonin synthesis genes. Furthermore, CRX(-) mice exhibit disruption of circadian rhythms. The human CRX gene maps to chromosome 19q13.3 within the region of the cone-rod dystrophy-2 locus (CORD2). Mutations in the CRX gene are implicated in the visual pathologies of CORD, Leber congenital amaurosis (LCA) and retinitis pigmentosa (RP). All characterized CRX gene mutations produce disease in heterozygotes although there is no known correlation between the pathologic phenotype and genetic mutation. Missense mutations of the CRX gene affect the homeobox domain, whereas frameshift mutations affect the OTX domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 166-285 mapping near the C-terminus of CRX of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 166-285 mapping near the C-terminus of CRX of human origin |
| Entrez Gene | 1406 |
|---|
Application
Western blot analysis of CRX expression in non-transfected: (A) and mouse CRX transfected: (B) whole cell lysates.
Application
Western blot analysis of CRX expression in non-transfected: (A) and mouse CRX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRX expression in Y79 (A) and ARPE-19 (B) whole cell lysates.
IF
(C) Immunocytochemistry analysis was used to detect cone-rod homeobox (Crx)-positive cells with different treatments (n = 3).| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
