Application
Western blot analysis of MafB expression in non-transfected: (A) and human MafB transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MafB expression in non-transfected: (A) and human MafB transfected: (B) CHO whole cell lysates.| Product Name | MafB Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 111-210 of MafB of human origin |
| Modification Notes | Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. c-Maf (Maf-2) and the closely related family members Neural retina leucine zipper (Nrl), L-Maf, and Krml1/MafB (Maf-1) all bind to T-MARE sites and have been implicated in a wide variety of developmental and physiologic roles. The three small Maf family proteins (MafF, MafG, and MafK) are components of NF-E2 that function as heterodimers with the large tissue-restricted subunit of NF-E2 called p45, and they are implicated in the transcriptional regulation of many erythroid-specific genes. MafB is expressed in a wide variety of tissues and encodes a protein containing a typical bZip motif in its carboxy-terminal region. As a transcriptional activator, MafB plays a pivotal role in regulating lineage-specific gene expression during hematopoiesis by repressing Ets-1-mediated transcription of key erythroid-specific genes in myeloid cells. c-Maf interacts with the c-Myb DNA binding domain and forms Myb-Maf complexes, which inturn mediate the cooperative interactions between c-Myb and Ets-1 during early myeloid cell differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of MafB of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-210 of MafB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-210 of MafB of human origin |
| Entrez Gene | 9935 |
|---|
Application
Western blot analysis of MafB expression in non-transfected: (A) and human MafB transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MafB expression in non-transfected: (A) and human MafB transfected: (B) CHO whole cell lysates.| Application Notes | recommended for detection of MafB of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
