Application
Western blot analysis of SRA expression in non-transfected: (A) and mouse SRA transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SRA expression in NIH/3T3 whole cell lysate.| Product Name | SRA Antibody (FL-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-220 representing full length SRA of mouse origin |
| Modification Notes | Steroid receptor RNA activator (SRA) selectively mediates transactivation of steroid hormone receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. There are six RNA motifs in SRA that are important for coactivation. SRA is ubiquitously expressed in normal tissues with higher levels of expression in liver and skeletal muscle. SRA is expressed at a low level in brain. SRA is expressed at higher levels in breast tumor than in normal tissue. Overexpression of SRA stimulates ERalpha transcriptional activity. In cells transfected with antisense oligodeoxynucleotides to SRA, ERalpha expression is reduced in a dose-dependent fashion (6). SMRT/HDAC1 associated repressor protein (SHARP) binds to SRA and inhibits SRA-potentiated steroid receptor transciption. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SRA of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-220 representing full length SRA of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-220 representing full length SRA of mouse origin |
| Entrez Gene | 10011 |
|---|
Application
Western blot analysis of SRA expression in non-transfected: (A) and mouse SRA transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SRA expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of SRA of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
