Application
Western blot analysis of c-Yes phosphorylation in non-transfected 293T: (A), mouse c-Yes transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-c-Yes Antibody (Tyr 537) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a short amino acid sequence containing phosphorylated Tyr 537 c-Yes of human origin |
| Modification | Phosphoralated |
| Modification Notes | Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that control a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Human c-Yes is the cellular homolog of the Yamaguchi sarcoma virus oncogene, Yes1. The human c-Yes gene maps to chromosome 18 and encodes a 543 amino acid protein. c-Src and c-Yes kinases are more than 80% homologous outside of unique amino-termini. Their respective SH3 and SH2 domains are capable of directing specificity in substrate binding. Human c-Yes is subject to autophosphorylation at Tyr 537. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Tyr 537 phosphorylated c-Yes of human origin, correspondingly phosphorylated Tyr 535 of mouse origin and correspondingly phosphorylated Tyr 536 of rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a short amino acid sequence containing phosphorylated Tyr 537 c-Yes of human origin |
|---|---|
| Tissue Specificity | raised against a short amino acid sequence containing phosphorylated Tyr 537 c-Yes of human origin |
| Entrez Gene | 7525 |
|---|
Application
Western blot analysis of c-Yes phosphorylation in non-transfected 293T: (A), mouse c-Yes transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Tyr 537 phosphorylated c-Yes of human origin, correspondingly phosphorylated Tyr 535 of mouse origin and correspondingly phosphorylated Tyr 536 of rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
