Application
Western blot analysis of MSK1 expression in HL-60 whole cell lysate.Application
Western blot analysis of phosphorylated MSK1 expression in HL-60 whole cell lysate.Application
Western blot analysis of MSK1 phosphorylation in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-MSK1 Antibody (Ser 360) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 360 phosphorylated MSK1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification | Phosphoralated |
| Modification Notes | The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms. Human MSK1 is subject to phosphorylation at specific amino acid residues, including Ser 360. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| See 1 citations for p-MSK1Antibody (Ser 360): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 360 phosphorylated MSK1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 360 phosphorylated MSK1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9252 |
|---|
Application
Western blot analysis of MSK1 expression in HL-60 whole cell lysate.
Application
Western blot analysis of phosphorylated MSK1 expression in HL-60 whole cell lysate.
Application
Western blot analysis of MSK1 phosphorylation in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | See 1 citations for p-MSK1Antibody (Ser 360): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
