Application
Western blot analysis of hnRNP L expression in non-transfected: (A) and mouse hnRNP L transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of hnRNP L expression in non-transfected: (A) and mouse hnRNP L transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of hnRNP L expression in non-transfected 293T: (A), human hnRNP L transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of hnRNP L expression in SHP-77 (A) and HeLa (B) whole cell lysates and MEG-01 (C), NIH/3T3 (D), Jurkat (E) and HeLa (F) nuclear extracts.Application
Western blot analysis of hnRNP L expression in MEG-01 (A), NIH/3T3 (B), Jurkat (C) and HeLa (D) nuclear extracts.Application
Western blot analysis of hnRNP L expression in SHP-77 whole cell lysate.| Product Name | hnRNP L Antibody (H-78) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation (1,2). They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II (1). There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome (2,3). The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm (4). hnRNP I, also designated polypyrimidine tract-binding protein (PTB), and its homolog hnRNP L bind to the 3’ end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV) (2,5,6). The human hnRNP I gene encodes a protein that is localized in the nucleoplasm (7-9). hnRNP L like hnRNP I, is also localized in the nucleoplasm (6,9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of hnRNP L of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 257-334 mapping within an internal region of hnRNP L of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 257-334 mapping within an internal region of hnRNP L of human origin |
| Entrez Gene | 3191 |
|---|
Application
Western blot analysis of hnRNP L expression in non-transfected: (A) and mouse hnRNP L transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of hnRNP L expression in non-transfected: (A) and mouse hnRNP L transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of hnRNP L expression in non-transfected 293T: (A), human hnRNP L transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of hnRNP L expression in SHP-77 (A) and HeLa (B) whole cell lysates and MEG-01 (C), NIH/3T3 (D), Jurkat (E) and HeLa (F) nuclear extracts.
Application
Western blot analysis of hnRNP L expression in MEG-01 (A), NIH/3T3 (B), Jurkat (C) and HeLa (D) nuclear extracts.
Application
Western blot analysis of hnRNP L expression in SHP-77 whole cell lysate.| Application Notes | recommended for detection of hnRNP L of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
