Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and extracellular localization.Application
Western blot analysis of human recombinant MMP-13.Application
Western blot analysis of MMP-13 expression in SCC-4 whole cell lysate.| Product Name | MMP-13 Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 242-471 of MMP-13 of human origin |
| Modification Notes | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen types I, II and III. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapid and effective remodeling of collagenous ECM takes place, such as fetal bone development and adult bone remodeling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MMP-13 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other MMP family members also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 242-471 of MMP-13 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 242-471 of MMP-13 of human origin |
| Entrez Gene | 4322 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and extracellular localization.
Application
Western blot analysis of human recombinant MMP-13.
Application
Western blot analysis of MMP-13 expression in SCC-4 whole cell lysate.| Application Notes | recommended for detection of MMP-13 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other MMP family members also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
