Application
Western blot analysis of phosphorylated PLC γ2 expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.| Product Name | p-PLC γ2 Antibody (Tyr 753) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 753 of PLC γ2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1, PLC ∂2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC ©2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR © chain), which bind to its C-terminal SH2 domain. PLC ©1 associates with Syk in B cells, but PLC ©2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC ©2. In addition, Btk can induce PLC ©2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 753 phosphorylated PLC γ2 of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 753 of PLC &gamma 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 753 of PLC γ2 of human origin |
| Entrez Gene | 5336 |
|---|
Application
Western blot analysis of phosphorylated PLC γ2 expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.| Application Notes | recommended for detection of Tyr 753 phosphorylated PLC γ2 of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
