Application
Antibodies tested include p-PYK2 (Tyr 402): (A, B, C) and PYK2 (N-19): (D, E, F).Application
Western blot analysis of PYK2 phosphorylation in non-transfected 293T: (A), untreated human PYK2 transfected 293T: (B) and lambda protein phosphatase treated human PYK2 transfected: (C) 293T whole cell lysates.Application
Western blot analysis of phosphorylated PYK2 expression in untreated (A) and PMA-treated (B) Jurkat whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-PYK2 Antibody (Tyr 402) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 402 of PYK2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | PYK2 (proline-rich tyrosine kinase 2), a putative member of the FAK family, exhibits 61% sequence identity with FAK within its kinase domain. Like FAK, PYK2 has been shown to be a cytoplasmic protein tyrosine kinase, which is a substrate for the intrinsic protein tyrosine kinase activity of pp60Src. PYK2 (also designated CAKb or RAFTK) is highly expressed in the central nervous system. PYK2 is rapidly phosphorylated on tyrosine residues in response to stimuli, which increases intracellular calcium levels and, in turn, activates members of the PKC family of kinases. Specifically, PYK2 is phosphorylated on Tyr 402 after stimulation with heregulin. This promotes the formation of a multiprotein complex that mediates the phosphorylation of p190 RhoGAP by Src. Activation of the PYK2 kinase leads to modulation of ion channel function and the activation of the MAPK signaling pathway. PYK2 also contains phosphorylation sites within the activation loop at Tyr 579 and Tyr 580 and on the potential Grb2-binding site at Tyr 881. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 402 phosphorylated PYK2 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 402 of PYK2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 402 of PYK2 of human origin |
| Entrez Gene | 2185 |
|---|
Application
Antibodies tested include p-PYK2 (Tyr 402): (A, B, C) and PYK2 (N-19): (D, E, F).
Application
Western blot analysis of PYK2 phosphorylation in non-transfected 293T: (A), untreated human PYK2 transfected 293T: (B) and lambda protein phosphatase treated human PYK2 transfected: (C) 293T whole cell lysates.
Application
Western blot analysis of phosphorylated PYK2 expression in untreated (A) and PMA-treated (B) Jurkat whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Tyr 402 phosphorylated PYK2 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
