Application
Western blot analysis of GKLF expression in non-transfected: (A) and human GKLF transfected: (B) 293T whole cell lysates.| Product Name | LKLF Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-60 mapping at the N-terminus of LKLF of human origin |
| Modification Notes | The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include erythroid Krupple-like transcription factor (EKLF), lung Krupple-like transcription factor (LKLF) and gut Krupple-like transcription factor (GKLF). EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arrest. In a developing mouse embryo, LKLF is necessary for normal tunica media formation and blood vessel stabilization. LKLF is also sufficient to program quiescence in T cells by negatively regulating the c-Myc-dependent pathway. The gene for human LKLF maps to chromosome 19p13.11-p13.13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of LKLF of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-60 mapping at the N-terminus of LKLF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-60 mapping at the N-terminus of LKLF of human origin |
| Entrez Gene | 10365 |
|---|
Application
Western blot analysis of GKLF expression in non-transfected: (A) and human GKLF transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of LKLF of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
