Application
Western blot analysis of Calponin 3 expression in non-transfected 293T: (A), mouse Calponin 3 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of Calponin 3 expression in SK-N-SH whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse skin frozen section showing cytoplasmic staining.Application
Western blot analysis of Calponin 3 expression in JEG-3 (A) and KNRK (B) whole cell lysates.| Product Name | Calponin 3 Antibody (H-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 275-329 mapping at the C-terminus of Calponin 3 of human origin |
| Modification Notes | Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Calponin 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 275-329 mapping at the C-terminus of Calponin 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 275-329 mapping at the C-terminus of Calponin 3 of human origin |
| Entrez Gene | 1266 |
|---|
Application
Western blot analysis of Calponin 3 expression in non-transfected 293T: (A), mouse Calponin 3 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of Calponin 3 expression in SK-N-SH whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse skin frozen section showing cytoplasmic staining.
Application
Western blot analysis of Calponin 3 expression in JEG-3 (A) and KNRK (B) whole cell lysates.| Application Notes | recommended for detection of Calponin 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
