Application
Western blot analysis of CYB5R3 expression in non-transfected: (A) and human CYB5R3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CYB5R3 expression in non-transfected: (A) and mouse CYB5R3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CYB5R3 expression in mouse placenta tissue extract.| Product Name | CYB5R3 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-60 mapping at the N-terminus of CYB5R3 of human origin |
| Modification Notes | CYB5R3 (NADH-cytochrome b5 reductase 3, diaphorase-1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism and, in erythrocytes, methemoglobin reduction. A serine residue at position 117 seems to only be found in persons of African origin. The allele frequency is 0.23 in African Americans. It is not found in Caucasians, Asians, Indo-Aryans or Arabs. This difference seems to have no effect on the enzyme activity. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1), in which the enzyme is only deficient in erythrocytes with a mild cyanosis; type 2 (HM2), in which the enzyme is completely deficient; and type 3 (HM3), where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied by mental retardation and neurological impairment. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYB5R3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-60 mapping at the N-terminus of CYB5R3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-60 mapping at the N-terminus of CYB5R3 of human origin |
| Entrez Gene | 1727 |
|---|
Application
Western blot analysis of CYB5R3 expression in non-transfected: (A) and human CYB5R3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CYB5R3 expression in non-transfected: (A) and mouse CYB5R3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CYB5R3 expression in mouse placenta tissue extract.| Application Notes | recommended for detection of CYB5R3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
