Application
Western blot analysis of CTCF expression in K-562 (A), Jurkat (B), HeLa (C) and CCRF-CEM (D) nuclear extracts.Application
Western blot analysis of CTCF expression in non-transfected: (A) and human CTCF transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of CTCF expression in non-transfected: (A) and mouse CTCF transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.Application
Western blot analysis of CTCF expression in K-562 nuclear extract.| Product Name | CTCF Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-280 mapping at the N-terminus of CTCF of human origin |
| Modification Notes | CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus. During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CTCF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-280 mapping at the N-terminus of CTCF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-280 mapping at the N-terminus of CTCF of human origin |
| Entrez Gene | 10664 |
|---|
Application
Western blot analysis of CTCF expression in K-562 (A), Jurkat (B), HeLa (C) and CCRF-CEM (D) nuclear extracts.
Application
Western blot analysis of CTCF expression in non-transfected: (A) and human CTCF transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of CTCF expression in non-transfected: (A) and mouse CTCF transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
Application
Western blot analysis of CTCF expression in K-562 nuclear extract.| Application Notes | recommended for detection of CTCF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
