Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular cells and lymphoid tissue.Application
Western blot analysis of MGMT expression in non-transfected: (A) and human MGMT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human MGMT expression in MCF7 whole cell lysate.Application
Western blot analysis of MGMT expression in non-transfected: (A) and human MGMT transfected: (B) 293 whole cell lysates.Application
Western blot analysis of MGMT expression in non-transfected 293: (A) and human MGMT transfected 293: (B) whole cell lysates and MCF7 nuclear extract (C).Application
Western blot analysis of MGMT expression in MCF7 nuclear extract.| Product Name | MGMT Antibody (FL-207) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-207 representing full length MGMT of human origin |
| Modification Notes | MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MGMT of human, and to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-207 representing full length MGMT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-207 representing full length MGMT of human origin |
| Entrez Gene | 4255 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular cells and lymphoid tissue.
Application
Western blot analysis of MGMT expression in non-transfected: (A) and human MGMT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human MGMT expression in MCF7 whole cell lysate.
Application
Western blot analysis of MGMT expression in non-transfected: (A) and human MGMT transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of MGMT expression in non-transfected 293: (A) and human MGMT transfected 293: (B) whole cell lysates and MCF7 nuclear extract (C).
Application
Western blot analysis of MGMT expression in MCF7 nuclear extract.| Application Notes | recommended for detection of MGMT of human, and to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
