Application
Western blot analysis of CYP4F12 expression in non-transfected: (A) and human CYP4F12 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CYP4F11 expression in non-transfected 293T: (A), human CYP4F11 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Product Name | CYP4F2 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-270 mapping within an internal region of CYP4F2 of human origin |
| Modification Notes | The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP4F isoforms are involved in the oxidation of important cellular mediators, such as leukotriene B4 (LTB4) and prostaglandins. CYP4F2 catalyzes omega-hydroxylation of LTB4 to a less potent proinflammatory eicosanoid, 20-OH-LTB4 as well as arachidonic acid. CYP4F2 is expressed in liver and kidney. Its expression is repressed by peroxisomal proliferators and induced by retinoic acid. X-linked adrenoleukodystrophy (X-ALD) is a severe neurodegenerative disorder biochemically characterized by elevated levels of very long-chain fatty acids (VLCFA). CYP4F2 participates in the omega-hydroxylation of VLCFAs, which may provide an alternative mode of treatment for X-ALD patients. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYP4F2 and, to a lesser extent, CYP4F11 of human origin by WB, IP, IF and ELISA may cross-react with other CYP4F family members also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-270 mapping within an internal region of CYP4F2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-270 mapping within an internal region of CYP4F2 of human origin |
| Entrez Gene | 8529 |
|---|
Application
Western blot analysis of CYP4F12 expression in non-transfected: (A) and human CYP4F12 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CYP4F11 expression in non-transfected 293T: (A), human CYP4F11 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of CYP4F2 and, to a lesser extent, CYP4F11 of human origin by WB, IP, IF and ELISA may cross-react with other CYP4F family members also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
