Application
Western blot analysis of Rap1GAP expression in non-transfected 293T: (A), human Rap1GAP transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of Rap1GAP expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.| Product Name | Rap1GAP Antibody (H-93) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 571-663 mapping at the C-terminus of Rap1GAP of human origin |
| Modification Notes | Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gå z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the å subunit, and also attenuates the ability of activated Gå z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gå z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rap1GAP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 571-663 mapping at the C-terminus of Rap1GAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 571-663 mapping at the C-terminus of Rap1GAP of human origin |
| Entrez Gene | 5909 |
|---|
Application
Western blot analysis of Rap1GAP expression in non-transfected 293T: (A), human Rap1GAP transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of Rap1GAP expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.| Application Notes | recommended for detection of Rap1GAP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
