Application
Western blot analysis of 20S Proteasome α1 expression in non-transfected 293T: (A), human 20S Proteasome α1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 20S Proteasome α1 expression in C4 (A), HeLa (B) and MIA PaCa-2 (C) whole cell lysates.Application
Western blot analysis of 20S Proteasome α1 expression in non-transfected: (A) and mouse 20S Proteasome α1 transfected: (B) 293T whole cell lysates.| Product Name | 20S Proteasome α1 Antibody (H-95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 131-225 mapping near the C-terminus of 20S Proteasome α1 of human origin |
| Modification Notes | Ubiquitin-dependent proteolysis mediates selective destruction of various cell cycle regulators, transcription factors and tumor suppressors. In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S proteasome. At specific stages of development, embryo- and tissue-specific components of the 26S proteasome form, facilitating proteolysis. 20S Proteasome å1, also designated macropain subunit C2 or PROS-30, is a prosomal protein involved in a non-lysosomal ATP/ubiquitin-dependent proteolytic pathway. The entire proteasome is composed of at least 15 non-identical subunits which form a highly-ordered ring-shaped structure. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 20S Proteasome α1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-225 mapping near the C-terminus of 20S Proteasome &alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-225 mapping near the C-terminus of 20S Proteasome α1 of human origin |
| Entrez Gene | 5682 |
|---|
Application
Western blot analysis of 20S Proteasome α1 expression in non-transfected 293T: (A), human 20S Proteasome α1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 20S Proteasome α1 expression in C4 (A), HeLa (B) and MIA PaCa-2 (C) whole cell lysates.
Application
Western blot analysis of 20S Proteasome α1 expression in non-transfected: (A) and mouse 20S Proteasome α1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of 20S Proteasome α1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
