Application
Western blot analysis of Thrombospondin 1/2 expression in Saos-2 (A) and 3T3-L1 (B) whole cell lysates.Application
Western blot analysis of Thrombospondin 1/2 expression in Saos-2 whole cell lysate.Application
Western blot analysis of Thrombospondin 1 expression in non-transfected control (A) and Thrombospondin 1 siRNA transfected (B) HeLa cells. Blot probed with Thrombospondin 1/2 (H-300): . α-actinin (H-2): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed CCD-1064-SK cells showing membrane staining.| Product Name | Thrombospondin 1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 401-700 of Thrombospondin 2 of human origin |
| Modification Notes | The thrombospondin proteins (TSP 1-4) compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Thrombospondins play a role in development, aggregation of platelets, adhesion and migration of cells, and progression of cells through the growth cycle. Thrombospondin 1 is released from platelets in response to Thrombin stimulation and is a transient component of the extracellular matrix of developing and repairing tissues. Thrombospondin 2 shares a high degree of homology with Thrombospondin 1 and is thought to have overlapping but unique functions. Thrombospondin 3 is a developmentally regulated heparin binding protein. Thrombospondin 4 is neuronally expressed and stimulates neurite outgrowth. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Thrombospondin 1 and Thrombospondin 2 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 401-700 of Thrombospondin 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 401-700 of Thrombospondin 2 of human origin |
| Entrez Gene | 7057 |
|---|
Application
Western blot analysis of Thrombospondin 1/2 expression in Saos-2 (A) and 3T3-L1 (B) whole cell lysates.
Application
Western blot analysis of Thrombospondin 1/2 expression in Saos-2 whole cell lysate.
Application
Western blot analysis of Thrombospondin 1 expression in non-transfected control (A) and Thrombospondin 1 siRNA transfected (B) HeLa cells. Blot probed with Thrombospondin 1/2 (H-300): . α-actinin (H-2): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed CCD-1064-SK cells showing membrane staining.| Application Notes | recommended for detection of Thrombospondin 1 and Thrombospondin 2 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
