Application
Western blot analysis of Neurogenin 3 expression in mouse pancreas tissue extract.| Product Name | Neurogenin 3 Antibody (M-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Neurogenin 3 of mouse origin |
| Modification Notes | The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the nuerogenin gene promoter. Neurogenin 3 is involved in the intial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, Neuro D, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of Neuro D in a bHLH cascade. Neurogenin 3 activates the expression of Neuro D at the onset of islet cell differentiation through box sequences E1 and E3 in the Neuro D promoter. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Neurogenin 3 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Neurogenin 3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Neurogenin 3 of mouse origin |
| Entrez Gene | 11925 |
|---|
Application
Western blot analysis of Neurogenin 3 expression in mouse pancreas tissue extract.| Application Notes | recommended for detection of Neurogenin 3 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
