Application
Western blot analysis of NGFR p75 expression in non-transfected 293T: (A), mouse NGFR p75 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed SK-N-MC cells showing membrane localization.Application
Western blot analysis of NGFR p75 expression in Hs 294T whole cell lysate immunoprecipitated with NGFR p75 (ME20.4): and detected with NGFR p75 (H-92): .| Product Name | NGFR p75 Antibody (H-92) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 266-357 mapping near the C-terminus of nerve growth factor receptor (NGFR) p75 of human origin |
| Modification Notes | The Trk oncogene encodes a membrane-spanning protein tyrosine kinase, gp140Trk, whose expression is restricted in vivo to neurons of the sensory spinal and cranial ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs with picomolar quantities of factor, indicating that the response is mediated by physiological amounts of NGF, suggesting that Trk A participates in the primary signal transduction mechanism of NGF. An additional component of the Trk A receptor complex, NGFR p75, binds to the neurotrophic factors with low affinity but is required for efficient signaling. NGFR p75 accelerates Trk A activation and may recruit downstream effector molecules to the liganded complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 266-357 mapping near the C-terminus of nerve growth factor receptor (NGFR) p75 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 266-357 mapping near the C-terminus of nerve growth factor receptor (NGFR) p75 of human origin |
| Entrez Gene | 4804 |
|---|
Application
Western blot analysis of NGFR p75 expression in non-transfected 293T: (A), mouse NGFR p75 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed SK-N-MC cells showing membrane localization.
Application
Western blot analysis of NGFR p75 expression in Hs 294T whole cell lysate immunoprecipitated with NGFR p75 (ME20.4): and detected with NGFR p75 (H-92): .| Application Notes | recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
