Application
Western blot analysis of SEC23 expression in non-transfected 293T: (A), human SEC23 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Product Name | SEC23 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of SEC23 isoform A subunit of the COPII complex of human origin |
| Modification Notes | COPII-coated vesicles form on the endoplasmic reticulum by the stepwise recruitment of three cytosolic components: Sar1-GTP to initiate coat formation, Sec23/24 heterodimer to select SNARE and cargo molecules, and Sec13/31 to induce coat polymerization and membrane deformation. Sec23A is the functional human counterpart of the yeast COPII component Sec23p which suggests that it plays a similar role in mammalian protein export from the ER. Mouse Sec23 is most abundant in brain and fibroblasts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SEC23 isoforms A and B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of SEC23 isoform A subunit of the COPII complex of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of SEC23 isoform A subunit of the COPII complex of human origin |
| Entrez Gene | 10483 |
|---|
Application
Western blot analysis of SEC23 expression in non-transfected 293T: (A), human SEC23 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of SEC23 isoforms A and B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
