Application
Western blot analysis of γ-GCSc expression in non-transfected: (A) and mouse γ-GCSc transfected: (B) 293T whole cell lysates.Application
Western blot analysis of γ-GCSc expression in non-transfected 293T: (A), human γ-GCSc transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.Application
Western blot analysis of γ-GCSc expression in Hep G2 (A) and A549 (B) whole cell lysates.| Product Name | γ-GCSc Antibody (H-338) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 338-637 mapping at the C-terminus of γ-GCSc of human origin |
| Modification Notes | The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of γ-GCSc of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 338-637 mapping at the C-terminus of &gamma -GCSc of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 338-637 mapping at the C-terminus of γ-GCSc of human origin |
| Entrez Gene | 2729 |
|---|
Application
Western blot analysis of γ-GCSc expression in non-transfected: (A) and mouse γ-GCSc transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of γ-GCSc expression in non-transfected 293T: (A), human γ-GCSc transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.
Application
Western blot analysis of γ-GCSc expression in Hep G2 (A) and A549 (B) whole cell lysates.| Application Notes | recommended for detection of γ-GCSc of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
