Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing basement membrane and cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of EMMPRIN expression in A-431 (A), SK-MEL-28 (B), WI 38 (C) and HeLa (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane localization.| Product Name | EMMPRIN Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 1-200 mapping of EMMPRIN of human origin |
| Modification Notes | Extracellular matrix metalloproteinase inducer (EMMPRIN), also designated basigin or CD147, is involved in the regulation of matrix remodeling at the epidermal-dermal interface. EMMPRIN stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of EMMPRIN of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-200 mapping of EMMPRIN of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-200 mapping of EMMPRIN of human origin |
| Entrez Gene | 682 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing basement membrane and cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of EMMPRIN expression in A-431 (A), SK-MEL-28 (B), WI 38 (C) and HeLa (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane localization.| Application Notes | recommended for detection of EMMPRIN of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
