Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PGI2 synthase expression in rat skeletal muscle tissue extract.| Product Name | PGI2 synthase Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-290 mapping near the N-terminus of Prostaglandin I2 synthase of human origin |
| Modification Notes | Prostacyclin (also known as prostaglandin I2) is a potent vasodilator and inhibitor of platelet aggregation (1). The enzyme PGI2 synthase (also known as prostacyclin synthase) catalyzes the isomerization of prostaglandin H2 (PGH2) to prostacyclin (1). Although it has absorbance spectral features characteristic of the cytochrome P450s, PGIS has no monooxygenase activity and does not require an external source of electrons to initiate its enzyme reaction (2). PGI2 synthase is the single member of family 8 of the cytochrome P450 superfamily (3). PGI2 synthase is a polypeptide of 500 amino acids with sequence homology to cholesterol 7-alpha-monooxygenase, a member of the CYP7 family of cytochrome P450s (4). The gene which encodes PGI2 synthase maps to human chromosome 20q13.11-q13.13 (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PGI2 synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-290 mapping near the N-terminus of Prostaglandin I2 synthase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-290 mapping near the N-terminus of Prostaglandin I2 synthase of human origin |
| Entrez Gene | 5740 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PGI2 synthase expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of PGI2 synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
