Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PRP8 expression in Y79 (A), K-562 (B) and Jurkat (C) whole cell lysates.| Product Name | PRP8 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2036-2335 mapping at the C-terminus of PRP8 of human origin |
| Modification Notes | PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5’ splice site (5’SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3’SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PRP8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2036-2335 mapping at the C-terminus of PRP8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2036-2335 mapping at the C-terminus of PRP8 of human origin |
| Entrez Gene | 10594 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing nuclear staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PRP8 expression in Y79 (A), K-562 (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of PRP8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
