Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of PLC β3 expression in non-transfected 293T: (A), mouse PLC β3 transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of PLC β3 expression in non-transfected 293T: (A), human PLC β3 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic and membrane staining of glandular cells and lymphoid tissue at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PLC β3 expression in A-431 (A), MCF7 (B), U-937 (C), NIH/3T3 (D) and KNRK (E) whole cell lysates.| Product Name | PLC β3 Antibody (H-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1151-1234 mapping at the C-terminus of PLC β3 of human origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. A total of eight mammalian PLC isozymes have been described (PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1 and PLC ∂2). The ©-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two ©-type enzymes, but not the ∫ and ∂ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC ∫1, PLC ∫2 and PLC ∫3 is mediated by the a subunits of the Gq class of heterotrimeric G proteins and by certain bg G protein subunits. The regulatory mechanisms for PLC ∂1 and PLC ∂2 are not yet resolved. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1151-1234 mapping at the C-terminus of PLC &beta 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1151-1234 mapping at the C-terminus of PLC β3 of human origin |
| Entrez Gene | 5331 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of PLC β3 expression in non-transfected 293T: (A), mouse PLC β3 transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of PLC β3 expression in non-transfected 293T: (A), human PLC β3 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic and membrane staining of glandular cells and lymphoid tissue at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PLC β3 expression in A-431 (A), MCF7 (B), U-937 (C), NIH/3T3 (D) and KNRK (E) whole cell lysates.| Application Notes | recommended for detection of PLC β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
