Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of NOS2 expression in uninduced (A) and LPS/IFN-γ stimulated (B) RAW 264.7 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.Application
Intracellular FCM analysis of fixed and permeabilized untreated (green line histogram) and LPS/PMA-treated (solid orange histogram) RAW 264.7 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .| Product Name | NOS2 Antibody (N-20) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of NOS2 of human origin |
| Modification Notes | Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of NOS2 (iNOS) of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of NOS2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of NOS2 of human origin |
| Entrez Gene | 4843 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of NOS2 expression in uninduced (A) and LPS/IFN-γ stimulated (B) RAW 264.7 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.
Application
Intracellular FCM analysis of fixed and permeabilized untreated (green line histogram) and LPS/PMA-treated (solid orange histogram) RAW 264.7 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .| Application Notes | recommended for detection of NOS2 (iNOS) of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
