Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of DRP1 expression in non-transfected: (A) and human DRP1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of DRP1 expression in non-transfected: (A) and human DRP1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | DRP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 560-736 mapping at the C-terminus of DRP1 of human origin |
| Modification Notes | Dynamin-related protein 1 (DRP1) mediates outer mitochondrial membrane fission in mammalian cells. DRP1 is also known as Dynamin-like protein 1, (Dlp1), DVLP or Dymple. DRP1 contains the N-terminal tripartite GTP-binding domain characteristic of the Dynamin superfamily of GTPases. DRP1 exists as a T-shaped dimer which contains a head, leg and stalk. The addition of GTP induces a rearrangement of the head and stalk that generates a force that ultimately results in membrane constriction. DRP1 is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain. In the cell, DRP1 localized to the perinuclear region. In mouse brain, DRP1 is highly expressed in the cerebellum with particularly high levels in cerebellar Purkinje cells. During apoptosis, DRP1 translocates from the cytosol to mitochondria and localizes to potential sites of organelle division. Cell death is averted upon DRP inhibition, suggesting a critical role for mitochondrial fission in apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of all DRP1 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 560-736 mapping at the C-terminus of DRP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 560-736 mapping at the C-terminus of DRP1 of human origin |
| Entrez Gene | 10059 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of DRP1 expression in non-transfected: (A) and human DRP1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of DRP1 expression in non-transfected: (A) and human DRP1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of all DRP1 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
