Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of NRAMP 1 expression in J774A.1 whole cell lysate.| Product Name | NRAMP 1 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 451-550 mapping at the C-terminus of NRAMP 1 of human origin |
| Modification Notes | Natural resistance associated macrophage proteins (NRAMPs) belong to a super-family of highly conserved integral membrane proteins. NRAMP 1 is an intracellular macrophage protein located at the phagosomal membrane where it functions as a divalent cation transporter for Fe2+, Zn2+ and Mn2+. NRAMP 1 is a pH-dependent antiporter that transports metal ions either into or out of the phagosome against a proton gradient. In humans, polymorphisms in the NRAMP 1 gene are linked to susceptibility to M. tuberculosis and leprosy. NRAMP 2 is another divalent cation transporter ubiquitously expressed as two splice variants, which are distinguished by the presence (isoform 1) or absence (isoform 2) of an iron response element. In the duodenum of the small intestine, dietary iron regulates NRAMP 2 expression at the brush border. Mutations in the gene for NRAMP 2 in mice and rats result in severe anemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NRAMP 1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 451-550 mapping at the C-terminus of NRAMP 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 451-550 mapping at the C-terminus of NRAMP 1 of human origin |
| Entrez Gene | 6556 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of NRAMP 1 expression in J774A.1 whole cell lysate.| Application Notes | recommended for detection of NRAMP 1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
