Application
Western blot analysis of Elmo1 expression in non-transfected 293T: (A), mouse Elmo1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Elmo expression in MOLT-4 (A) and Jurkat (B) whole cell lysates.| Product Name | Elmo Antibody (FL-727) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-727 representing full length Elmo1, isoform 1 of human origin |
| Modification Notes | Elmo (engulfment and cell motility) proteins share similarity to C. elegans CED-12. The C. elegans genes ced-2, ced-5, ced-10, and ced-12 and their mammalian homologs CRKII, DOCK1, RAC1 and ELMO mediate cytoskeletal rearrangements during phagocytosis of apoptotic cells and cell motility. Elmo1 associates with DOCK 180 and may influence phagocytosis and effect cell shape changes. Src family kinase mediated tyrosine phosphorylation of ELMO1 influences signaling through Elmo1/Crk/DOCK 180 pathways. Elmo2 interacts directly with Rho G in a GTP-dependent manner and forms a ternary complex with DOCK 180 to induce activation of Rac 1. The RhoG-Elmo2-DOCK 180 pathway is required for activation of Rac 1 and cell spreading mediated by integrin, as well as for neurite outgrowth induced by nerve growth factor. Elmo3 acts in assocation with DOCK180 and Crk II and may be required in complex with DOCK180 to activate Rac/Rho small GTPases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of a broad range of Elmo proteins of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-727 representing full length Elmo1 isoform 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-727 representing full length Elmo1, isoform 1 of human origin |
| Entrez Gene | 9844 |
|---|
Application
Western blot analysis of Elmo1 expression in non-transfected 293T: (A), mouse Elmo1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Elmo expression in MOLT-4 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of a broad range of Elmo proteins of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
