Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of utrophin expression in L6 whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of human recombinant utrophin fusion protein.| Product Name | utrophin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 801-1100 mapping near the C-terminus of utrophin of human origin |
| Modification Notes | Dystrophin and utrophin are related structural, Actin-binding proteins that are involved in anchoring the cytoskeleton to the plasma membrane (1,2). Dystrophin is the protein product of the Duchenne/Becker muscular dystrophy gene (3). Dystrophin expression is found in muscle and brain tissues, where it is localized to the inner surface of the plasma membrane (3). It has been speculated that alternative splicing of the carboxy terminus allows dystrophin to interact with a variety of proteins (4,5). Research has shown that the loss of dystrophin-associated proteins in Duchenne afflicted muscle is due to the absence of dystrophin rather than to muscle degradation and that the lack of dystrophin results in the loss of linkage between the cytoskeleton and the extracellular matrix (6). Evidence suggests that the upregulation of utrophin can reduce the dystrophic pathology (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of utrophin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 801-1100 mapping near the C-terminus of utrophin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 801-1100 mapping near the C-terminus of utrophin of human origin |
| Entrez Gene | 7402 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of utrophin expression in L6 whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of human recombinant utrophin fusion protein.| Application Notes | recommended for detection of utrophin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
